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Trypanosoma vivax: a novel method for purification from experimentally infected sheep blood.

González LE, García JA, Núñez C, Perrone TM, González-Baradat B, Gonzatti MI, Reyna-Bello A

Universidad Simón Bolívar, Departamento de Biología Celular, Grupo deBioquímica e Inmunología de Hemoparásitos, Caracas, Venezuela.

Trypanosoma vivax is the principal etiological agent of bovine trypanosomosis, a widely disseminated disease in tropical and subtropical regions. Here, we present a simple and reproducible method for the purification of T. vivax from experimentally infected and immunosuppressed sheep, using an isopycnic Percoll gradient, followed by DEAE-cellulose chromatography, with an estimated yield of 11-15%. This method could be used for the purification of T. vivax geographical isolates from various locations and from different natural hosts.

Published 13 September 2005 in Exp Parasitol, 111(2): 126-9.
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